Chemical Reagents for Protein Modification, Third Edition

✍ Scribed by Roger L. Lundblad

• Publisher: CRC Press
• Year: 2004
• Tongue: English
• Leaves: 313
• Edition: 2
• Category: Library

✦ Synopsis

Revised and updated, Chemical Reagents for Protein Modification, Third Edition is an encyclopedic work describing the many approaches to the site-specific modification of proteins. More than 2,000 references are cited describing the development of the various reagents. This book explores how the use of site-specific protein modification relates to a wide variety of biotechnology processes and products, such as protein microarrays, hydrogels, controlled-release pharmaceuticals, and biotherapeutics, and proteomics.With chapters focusing on analyses of individual amino acids, additional chapters cover chemical cross-linking and the chemical cleavage of peptide chains. There is considerable reference to the use of site-specific chemical modification in proteomics. The author pays particular attention to the general environmental factors that influence the reactivity of individual amino acid residues. He presents techniques for the characterization of modified proteins, including mass spectrometry, and includes increased coverage of spectroscopic techniques and crystallographic analyses. This is followed by coverage of nitrosylation reactions and other non-enzymatic modification processes. Specific reference is given to the differential labeling of protein functional groups with reagents labeled with stable isotopes. Resources include a list of current journals that publish site-specific chemical modification studies, the most commonly used reagents and their properties, and a contact list of reagent suppliers.Chemical Reagents for Protein Modification, 3rd Edition presents the most frequently used methods for the site-specific chemical modification of proteins, techniques for protein characterization, precise laboratory data for factors that influence reactivity and reproducibility, and industry-specific resources.

✦ Table of Contents

Cover......Page 1
Chemical Reagents for Protein Modificaiton, Third Edition......Page 3
ISBN-13: 9780849319839......Page 4
Preface......Page 5
Acknowledgments......Page 6
Author......Page 9
Contents......Page 7
1
The Site-Specific
Chemical Modification
of Proteins......Page 10
REFERENCES......Page 23
2
The Modification
of Amino Groups......Page 40
REFERENCES......Page 65
3
The Modification of
Histidine Residues......Page 75
REFERENCES......Page 95
4
The Modification
of Arginine......Page 102
REFERENCES......Page 123
5
The Modification of
Carboxyl Groups......Page 129
REFERENCES......Page 141
6
The Modification
of Cysteine......Page 145
REFERENCES......Page 183
7
The Modification
of Cystine......Page 198
MATERIALS......Page 203
NOTES......Page 204
REFERENCES......Page 205
8
The Modification
of Methionine......Page 210
REFERENCES......Page 214
9
The Modification
of Tyrosine......Page 217
REFERENCES......Page 242
10
The Chemical
Modification
of Tryptophan......Page 251
REFERENCES......Page 267
11
The Chemical Cleavage
of Peptide Bonds......Page 273
REFERENCES......Page 282
12
The Chemical
Cross-Linking
of Peptide Chains......Page 288
REFERENCES......Page 297
Some Reagents Used in the Modification of Proteins......Page 303
STANDARD......Page 306
STANDARD......Page 307
STANDARD......Page 308
REFERENCES......Page 309