Chemical quantitation of hemoglobin glycosylation: Fluorometric detection of formaldehyde released upon periodate oxidation of glycoglobin
✍ Scribed by Paul M. Gallop; Rudolf Flückiger; Anne Hanneken; Michael M. Mininsohn; Kenneth H. Gabbay
- Book ID
- 102983538
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 503 KB
- Volume
- 117
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A sensitive fluorometric method for the quantitation of hemoglobin glycosylation, based upon periodate oxidation of the carbohydrate moieties present on both the (Y-and c-amino groups of globin is described. The formaldehyde product is measured as the fluorescent 3,5diacetyl-1,4-dihydrolutidine formed from the condensation of formaldehyde with acetylacetone and ammonia.
This method is rigorously designed to assay glycosylated hemoglobin levels and to give a direct measure of the number of glycogroups per milligram of hemoglobin. It requires only 1 mg of protein and may also be used to determine the extent of the nonenzymatic glycosylation of other proteins.