Chemical mutagenesis testing in Drosophila. VI. Interlaboratory comparison of mutagenicity tests after treatment of larvae
✍ Scribed by R. Valencia; J. M. Mason; S. Zimmering
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 505 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0893-6692
No coin nor oath required. For personal study only.
✦ Synopsis
Previous publications on the National Toxicology Program (NTP)-sponsored mutagenicity testing program in Drosophila dealt with evaluations of chemicals fallowing adult treatment (feed, injection). The current paper deals with a comparison between the laboratories at Brown University (BRU) and the Universityof Wisconsin at Madison (UWM) regarding the response of larvae to treatment with chemicals in the sex-linked recessive lethal (SLRL) test and, where appropriate, the reciprocal translocation test 0 s well. Dimethylnitrosamine (DMN) and dimethylbenz(o)anthracene were used first as reference mutagens. Six coded compounds were then evaluated regording their repeatability in the two laboratories; the compounds were benzo(a)pyrene, 3-methylcholanthrene, coumarin, quinoline, formaldehyde, and 9-aminoacridine. It was concluded that at this time it would be imprudent to forgo larval treatment in cases where compounds proved negative after adult feeding. Accordingly, testing o series of 20 compounds negative after adult treatment is in progress.