Chemical ionization mass spectra of high molecular weight, biologically active compounds produced following supercritical fluid chromatography
✍ Scribed by H. T. Kalinoski; B. W. Wright; R. D. Smith
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 242 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1076-5174
No coin nor oath required. For personal study only.
✦ Synopsis
The principal applications of supercritical fluid chromatography (SFC) are for compounds that are thermally labile, of low volatility, of moderate polarity, or present in mixtures too complex for adequate separation using HPLC. Many compounds of biological and pharmaceutical interest are defined by these criteria. Recently, applications of SFC and SFC-MS to the determination of lower molecular weight compounds with biological activity were described (1,2).
The combination of SFC with mass spectrometry provides for sensitive detection with an added dimension of selectivity for the identification of compounds that are not chromatographically resolved. Mass spectrometry performance in SFC-MS is largely unaffected by polar solvents used as mobile phase modifiers that are needed for elution of more polar biological molecules. However, there are limitations on the types of samples that are amenable to SFC-MS analysis due to the inability to transport polar molecules of higher molecular weight and lower volatility through the SFC-MS interface (2,3). Additional problems can be encountered by limitations imposed on sample size and concentration when using split injection with capillary SFC-MS.