## Abstract Chemical cross‐linking of proteins, an established method in protein chemistry, has gained renewed interest in combination with mass spectrometric analysis of the reaction products for elucidating low‐resolution three‐dimensional protein structures and interacting sequences in protein c
Chemical cross-linking and FTICR mass spectrometry for protein structure characterization
✍ Scribed by Andrea Sinz
- Book ID
- 105889052
- Publisher
- Springer
- Year
- 2004
- Tongue
- English
- Weight
- 251 KB
- Volume
- 381
- Category
- Article
- ISSN
- 1618-2650
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📜 SIMILAR VOLUMES
A general method of unequivocally identifying and obtaining sequence information on cross-linked peptides derived by proteolytic digestion of cross-linked proteins has been developed. The method is based on isotopic labeling of ␣-amino groups with 2,4-dinitrofluorobenzene (DNFB) coupled with electro
## Abstract | I. | Introduction | 163 | | II. | Photochemical Cross‐Linking | 165 | | III. | Structural Analysis of Protein–Nucleic Acid Heteroconjugates | 165 | | IV. | Analytical Challenges | 168 | | | A. Ionization of Peptide–Oligonucleotide Heteroconjugates | 168 | | | 1. MALDI MS Analysi