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Chemical and Enzymatic Synthesis of Lactose Operator of Escherichia coli and its Binding to Lactose Repressor

✍ Scribed by Chander P. Bahl, Ray Wu, Keichi Itakura, Nobuya Katagiri and Saran A. Narang


Book ID
123653531
Publisher
National Academy of Sciences
Year
1976
Tongue
English
Weight
741 KB
Volume
73
Category
Article
ISSN
0027-8424

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Equilibrium binding of Escherichia coli LexA repressor to the recA operator was studied by the polyacrylamide gel mobility shift assay as a function of solution conditions. In the presence of NsCl at 20"C, there was a significant salt dependence in binding to the recA operator, typical for protein-n