Charge microheterogeneity of the β-trace proteins (lipocalin-type prostaglandin D synthase) in the cerebrospinal fluid of patients with neurological disorders analyzed by capillary isoelectrofocusing
✍ Scribed by Atsushi Hiraoka; Kohsuke Seiki; Hiroshi Oda; Naomi Eguchi; Yoshihiro Urade; Itaru Tominaga; Kohji Baba
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 73 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
Charge microheterogeneity of the â-trace proteins (lipocalin-type prostaglandin D synthase) in the cerebrospinal fluid of patients with neurological disorders analyzed by capillary isoelectrofocusing
Charge microheterogeneity of the b-trace protein (b-TP = lipocalin-type prostaglandin D synthase) in the cerebrospinal fluid (CSF) of patients with various neurological disorders was analyzed by capillary isoelectric focusing (CIEF). Under the conditions employed, b-TP in the low-molecular-weight protein fraction of CSF was separated into at least four isoforms with different pI values. An isoform with the pI value of 4.6-4.8 was usually the most abundant. The total b-TP level in the CSF was determined by enzyme-linked immunosorbent assay (ELISA) to be elevated in patients recovering from organic damage to the CNS and those with pathological brain atrophy. Changes in the total b-TP level in the CSF were occasionally accompanied by those in its charge microheterogeneity, as revealed by CIEF. Such quantitative and qualitative changes in b-TP in human CSF indicated changes in its pathophysiological roles in association with various neurological disorders.