Characterizing Structure and Activity of Subtilisin Enzyme in Nonaqueous Media with Molecular Dynamics Simulations
โ Scribed by Auh, Eugene; Ham, Sihyun
- Book ID
- 122323739
- Publisher
- Biophysical Society
- Year
- 2010
- Tongue
- English
- Weight
- 63 KB
- Volume
- 98
- Category
- Article
- ISSN
- 0006-3495
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๐ SIMILAR VOLUMES
## Abstract A protein solubilization method has been developed to directly solubilize protein clusters into organic solvents containing small quantities of surfactant and trace amounts of water. Termed โdirect solubilization,โ this technique was shown to solubilize three distinct proteinsโsubtilisi
## Abstract The phenomenon known as โligand imprintingโ or โligandโinduced enzyme memoryโ was first reported in 1988, when Russell and Klibanov observed that lyophilizing subtilisin in the presence of competitive inhibitors (that were subsequently removed) could significantly enhance its activity i