Characterization of β2-glycoprotein I-dependent and -independent “antiphospholipid” antibodies from lupus-prone NZW/BXSB F1 hybrid male mice
✍ Scribed by Thiagarajan, Perumal; Le, Anhquyen; Shapiro, Sandor S.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 156 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0361-8609
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✦ Synopsis
Male (NZW × BXSB)F1 (W/BF1
) mice develop a systemic lupus-like syndrome characterized by thrombocytopenia, coronary vascular disease, nephritis, and anticardiolipin antibodies. Three stable hybridoma cell lines secreting monoclonal anticardiolipin antibodies were developed from these mice by fusing their splenic lymphocytes with nonsecreting myeloma cell line, NS-1. Monoclonal antibody A1.17 reacted with cardiolipin in a  2 -Glycoprotein I-dependent manner. The epitope for this antibody consisted of  2glycoprotein I bound to cardiolipin or immobilized on plastic plates. Other anionic phospholipid-binding proteins, such as prothrombin or annexin V, had no significant effect in the reactivity of these antibodies. The specificity is similar to the autoimmune anticardiolipin antibodies described in patients with systemic lupus erythematosus and other infectious diseases. In contrast, monoclonal antibodies A1.72 and A1.84 reacted with cardiolipin in the absence of  2 -glycoprotein I.  2 -glycoprotein I, either in the fluid phase or bound to cardiolipin, inhibited the binding of these antibodies. The specificity of the latter two antibodies was similar to that described in patients with syphilis and allied disorders. Both types of antibodies had lupus anticoagulant properties. Thus lupusprone male (NZW × BXSB)F1 (W/BF1) mice develop both  2 -glycoprotein I-dependent and  2 -glycoprotein I-independent anticardiolipin antibodies. Am.