Characterization of two human monoclonal IgM antibodies that recognize nuclear lamins

✍ Scribed by KaïS Lassoued; Chantal André; Francoise Danon; Robert Modigliani; Daniel Dhumeaux; Jean-Pierre Clauvel; Jean-Claude Brouet; Jean-Claude Courvalin

• Book ID: 102822819
• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 502 KB
• Volume: 22
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830220630

✦ Synopsis

Abstract

Using immunofluorescence and immunoblotting techniques, we have identified monoclonal IgM λ from two patients that are specific for lamins A and C and lamin B, respectively. Lamins A, B, and C are peripheral membrane proteins of the nuclear envelope with structural similarities to cytoplasmic intermediate filament proteins. When studiedby indirect immunofluorescence on rat tissues, the serum containing anti‐laminB IgM stained smooth and striated muscles in addition to nuclear envelopes. Lamin B antibodies affinity purified from this serum were able to label muscle cells, suggesting that lamin B shares an epitope(s) with an unidentified muscular component(s). Since in an enzyme‐linked immunosorbent assay there was no reactivity with a panel of proteins which are frequent targets of “natural” antibodies, these monoclonal IgM appear to belong to the rare category of IgM that possess a restricted specificity.