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Characterization of two genes, ARGRI and ARGRIII required for specific regulation of arginine metabolism in yeast

✍ Scribed by Dubois, Evelyne ;Bercy, Josiane ;Messenguy, Francine

Publisher: Springer
Year: 1987
Tongue: English
Weight: 657 KB
Volume: 207
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00331501

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✦ Synopsis

Three unlinked genes ARGRI, ARGRII and ARGIII are necessary to control the synthesis of the arginine anabolic and catabolic genes. The three genes have been cloned and sequenced and we report here the results for the ARGRI and ARGRIII genes. They encode proteins of 177 and 355 amino acids, respectively. The ARGRIII protein has a very acidic carboxy-terminus (17 aspartate residues). From a comparison of the sequences, the ARGRI and ARGRIII gene products do not show the common characteristics of other DNA binding proteins (nuclear localization and putative DNA binding site) in contrast to the ARGRII regulatory protein. The transcription of both genes is not affected by the presence of arginine in the growth medium.

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