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Characterization of Trophoblast-Reactive Monoclonal Antibodies by Flow Cytometry and Their Application for Fetal Cell Isolation

✍ Scribed by DORIS B. TSE; PATRICK ANDERSON; SIMON GOLDBARD; ALLEN M. GOWN; CATHERINE S. HAWES; ALAN DONNENFELD


Book ID
119871283
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
975 KB
Volume
731
Category
Article
ISSN
0890-6564

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A new method to quantify red blood cells (RBCs) containing fetal hemoglobin (HbF), or F cells, by flow cytometry was developed. The use of formaldehyde as fixative agent and sodium dodecyl sulfate (SDS) to permeabilize fixed RBCs resulted in a low staining background, a negligible Hb leakage, and mi