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Characterization of the smtA gene encoding an S-adenosylmethionine-dependent methyltransferase of Escherichia coli

โœ Scribed by Kunitoshi Yamanaka; Teru Ogura; Hironori Niki; Sota Hiraga


Book ID
109320819
Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
395 KB
Volume
133
Category
Article
ISSN
0378-1097

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Cloning and characterization of the prs
โœ Hove-Jensen, Biarne ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Springer ๐ŸŒ English โš– 865 KB

The gene, prs, encoding phosphoribosylpyrophosphate (PRPP) synthetase of Escherichia coli was isolated from a library of E. coli genes cloned in the bacteriophage 2D69 vector. A strain with a temperature-lethal defect in PRPP synthetase, (prs-2), was used as the host and cloning was performed by lys