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Characterization of the replication of a baculovirus mutant lacking the DNA polymerase gene

✍ Scribed by Adam L. Vanarsdall; Kazuhiro Okano; George F. Rohrmann

Book ID
113946169
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
311 KB
Volume
331
Category
Article
ISSN
0042-6822

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The cloned DNA polymerase I gene has been used to map the POL1 locus on the left arm of chromosome XIV, between MET4 and TOP2. Temperature-sensitive mutants in POL1 have been obtained by in vitro mutagenesis of the cloned gene and in vivo replacement of the wild-type allele with the mutated copy. Ph