Characterization of the osteoblast-like cell phenotype under microgravity conditions in the NASA-approved rotating wall vessel bioreactor (RWV)
✍ Scribed by Nadia Rucci; Silvia Migliaccio; Bianca Maria Zani; Anna Taranta; Anna Teti
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 419 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
Abstract
Weightlessness induces bone loss in humans and animal models. We employed the NASA‐approved Rotating Wall Vessel bioreactor (RWV) to develop osteoblast‐like cell cultures under microgravity and evaluate osteoblast phenotype and cell function. Rat osteoblast‐like cell line (ROS.SMER#14) was grown in the RWV at a calculated gravity of 0.008__g__. For comparison, aliquots of cells were grown in conventional tissue culture dishes or in Non‐Rotating Wall Vessels (N‐RWV) maintained at unit gravity. In RWV, osteoblasts showed high levels of alkaline phosphatase expression and activity, and elevated expression of osteopontin, osteocalcin, and bone morphogenetic protein 4 (BMP‐4). In contrast, the expression of osteonectin, bone sialoprotein II and BMP‐2 were unaltered compared to cells in conventional culture conditions. These observations are consistent with a marked osteoblast phenotype. However, we observed that in RWV osteoblasts showed reduced proliferation. Furthermore, DNA nucleosome‐size fragmentation was revealed both morphologically, by in situ staining with the Thymine‐Adenine binding dye bis‐benzimide, and electrophoretically, by DNA laddering. Surprisingly, no p53, nor bcl‐2/bax, nor caspase 8 pathways were activated by microgravity, therefore the intracellular cascade leading to programmed cell death remains to be elucidated. Finally, consistent with an osteoclast‐stimulating effect by microgravity, osteoblasts cultured in RWV showed upregulation of interleukin‐6 (IL‐6) mRNA, and IL‐6 proved to be active at stimulating osteoclast formation and resorbing activity in vitro. We conclude that under microgravity, reduced osteoblast life span and enhanced IL‐6 expression may result in inefficient osteoblast‐ and increased osteoclast‐activity, respectively, thus potentially contributing to bone loss in individuals subjected to weightlessness. J. Cell. Biochem. 85: 167–179, 2002. © 2002 Wiley‐Liss, Inc.