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Characterization of the O-polysaccharide of Pasteurella haemolytica serotype A1

✍ Scribed by Wayne B. Severn; James C. Richards

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 658 KB
Volume: 240
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(93)84190-h

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✦ Synopsis

Lipopolysaccharide was isolated from Pasteurella haemolyticu biotype A, serotype 1 by using the phenol-water extraction procedure. Hydrolysis with mild acid afforded a high-molecular-weight antigenie O-chain. On the basis of 1D and 2D NMR spectral studies and microanalytical chemical methods, the 0-polysaccharide was determined to be a linear polymer of a trisaccharide repeating unit having the structure --f 3)-P-o-Galp-(I + 3).P-o-GalpNAc-(1 + 4)-P-o-Galp-(I * This 0-polysaccharide antigen is expressed by several P. haemolytica biotype A serotypes.

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