A radiolabeled ATP assay was developed for measuring carboxylic acid:CoA ligase activity. The assay was designed to measure the formation of [gamma-33P]pyrophosphate from [gamma-33P]ATP in the course of the reaction. The assay was linear with protein concentration, and rates as low as 1 pmol/min wer
Characterization of the monovalent and divalent cation requirements for the xenobiotic carboxylic acid: CoA ligases of bovine liver mitochondria
β Scribed by Donald A Vessey; Michael Kelley
- Book ID
- 114149549
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 122 KB
- Volume
- 1382
- Category
- Article
- ISSN
- 0167-4838
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## Abstract The purification of xenobiotic/mediumβchain fatty acid:CoA ligases (XMβligases) from human liver mitochondria resulted in the isolation of two chromatographically separable forms (HXMβA and HXMβB). These two forms were purified to near homogeneity, cleaved with cyanogen bromide, the res