Characterization of the major protein fraction carmin from safflower seeds (Carthamus tinctorius L.) by dynamic light scattering and circular dichroism spectroscopy
✍ Scribed by Zirwer, D. ;Prakash, V. ;Raab, B. ;Schwenke, K. D. ;Gast, K.
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 280 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0027-769X
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✦ Synopsis
Hydrodynamic parameters of the major protein fraction carmin from safflower seed were determined by means of dynamic light scattering. The results indicated a translational diffusion coefficient of (3.81 f 0.06) x cm2 s-l and a STOKES radius of (5.6 f 0.06) nm. The calculated molecular mass M,, was (3.1 f 0.1)
x lo5 g/mol and the frictional ratio was 1.25 & 0.05. Evaluation of secondary structure from CD spectra using the method of PROVENCHER and GL~CKNER gave 8 % a-helix, 45 % 8-sheet and 47 % aperiodic structure.
These results are discussed in view of structural homology of oilseed proteins.