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Characterization of the Major Glutathione Transferases in Carp Liver

✍ Scribed by Elia, Antonia Concetta; Mantilacci, Luciana; Taticchi, Maria Illuminata; Principato, Giovanni

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
97 KB
Volume
284
Category
Article
ISSN
0022-104X

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✦ Synopsis

In fish, glutathione S-transferases are very important in the detoxification of water-borne pollutants. Carp, which often live and thrive under extreme conditions of water pollution, have been recommended by the European Community as an ideal species to test for the effect of various chemical substances often found in the aqueous environment. In this paper we describe the purification and kinetic characterization of the three major forms of glutathione Stransferase in carp liver cytosol. The main step for purification was affinity chromatography on Shexylglutathione-Sepharose 6B and the isoenzymes have been separated by isoelectric focusing at pH 7.7, 7.3, 7.1. Values of Km and kcat/Km for both reduced glutathione and 1-chloro-2,4-dinitrobenzene as substrates have been determined, and the isoenzymes characterized with several substrates and inhibitors.

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