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Characterization of the Lanthanide Ion-Binding Properties of Calcineurin-B Using Laser-Induced Luminescence Spectroscopy

✍ Scribed by Burroughs, Sarah E.; Horrocks, William D.; Ren, Hao; Klee, Claude B.


Book ID
126197083
Publisher
American Chemical Society
Year
1994
Tongue
English
Weight
1023 KB
Volume
33
Category
Article
ISSN
0006-2960

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A direct spectroscopic method for the determination of the submicromolar dissociation constant of Eu3'. ATP using laser-induced Eu3+ ion luminescence spectroscopy is described. The dissociation constant of Mg2+ .ATP is then determined by the competition of Mg2+ with Eu3+ for the binding of ATP. The