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Characterization of the Hf-NTA complex

✍ Scribed by S. K. Das; R. K. Chatterjee; S. K. Saha


Book ID
110332974
Publisher
Springer
Year
2002
Tongue
English
Weight
24 KB
Volume
251
Category
Article
ISSN
1588-2780

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Development and characterization of Ni-N
✍ Sabine A. Lauer; John P. Nolan 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 315 KB

## Abstract ## Background For ease of purification, proteins are often expressed with a short affinity sequence of five or six adjacent histidine residues (His‐tag). This His‐tag binds to the metal of metal chelator complexes such as Ni^2+^‐nitrilotriacetic acid (Ni‐NTA) or ‐iminodiacetic acid (Ni