Characterization of the gene encoding the 10 kDa polypeptide of photosystem II fromArabidopsis thaliana
✍ Scribed by Gabriel Gil-Gómez; Pedro F. Marrero; Diego Haro; José Ayté; Fausto G. Hegardt
- Book ID
- 104618811
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 395 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0167-4412
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✦ Synopsis
We report the characterization of a 1388 bp genomic fragment from Arabidopsis thaliana that encompasses the entire transcription unit of the gene encoding the precursor of 10 kDa polypeptide of photosystem II, 495 bp of the 5' flanking region and 73 bp of the 3' boundary of the gene. The deduced protein shows 78% and 73% homology, respectively, with its homologues from potato and spinach. The transcription of the gene seems to be greatly enhanced by light and only transcribed in substantial amounts in leaves and stems. Analysis of the putative 5' regulatory region of the gene shows homology with several cis-elements involved in light regulation of the transcription.
📜 SIMILAR VOLUMES
A cDNA encoding the precursor to a major 20-kDa thylakoid polypeptide of Chlamydomonas reinhardtii (P22), previously localized to the photosystem I light-harvesting complex (LHCI), was characterized. N-terminal sequencing of P22 identified the precursor cleavage site. Genomic Southern blots and poly