Characterization of the complex formation of 1,6-anhydro-β-maltotriose with potassium using 1H and 39K NMR spectroscopy

✍ Scribed by Takayuki Kato; Koji Tsubono; Osamu Kamo; Toshiyo Kato; Kazuo Furihata; Takashi Fujimoto; Tomoya Machinami; Mitsuru Tashiro

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 233 KB
• Volume: 47
• Category: Article
• ISSN: 0749-1581
• DOI: 10.1002/mrc.2495

✦ Synopsis

Abstract

The ^1^H and ^39^K longitudinal relaxation times (T~1~) and ^1^H diffusion coefficients were measured to investigate the complex formation of 1,6‐anhydro‐β‐maltotriose and potassium ions. Although the ^1^H‐T~1~ values of H3′, H5′, H1″ and H4″ decreased in the presence of potassium ions, ^1^H chemical shifts and ^1^H diffusion coefficients did not show significant changes. The long‐range coupling constants of ^3^J~C−H~ around the glycosyl bonds did not show significant changes either. In the measurements of ^39^K spectra, the ^39^K signal obviously broadened and the ^39^K‐T~1~ values decreased in the presence of 1,6‐anhydro‐β‐maltotriose, indicating the complex formation of 1,6‐anhydro‐β‐maltotriose and potassium ions. These results indicate that the conformation and molecular volume were unaffected in the complex formation. Copyright © 2009 John Wiley & Sons, Ltd.