Characterization of the cis-acting elements of the mouse myelin P2 promoter

✍ Scribed by V. A. Bharucha; K. W. C. Peden; B. R. Subach; V. Narayanan; G. I. Tennekoon

• Book ID: 102909632
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 909 KB
• Volume: 36
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490360503

✦ Synopsis

Abstract

Myelin P~2~ is a basic protein of an apparent molecular weight of 14,800. Expression of P~2~ has been found largely in the cytosol of Schwann cells in the peripheral nervous system. Although the function of P~2~ is unknown, its striking homology to a family of fatty acid binding proteins has led to the idea that P~2~ may function as a fatty acid transport molecule. To investigate the DNA elements that control the expression of P~2~, sequences 5′ to the coding region were cloned upstream of the cat reporter gene. A series of 3′ and 5′ promoter mutants was constructed and their activity determined following transfection into secondary Schwann cells and the MT~4~H1 Schwann cell line. Using this strategy, we have identified a 217 bp silencer region and a 142 bp positive regulatory region. In addition, we have localized the 5′ flanking sequences in the promoter that are responsive to cAMP induction and to the transcription factor CCAAT/enhancer binding protein (C/EBP). © 1993 Wiley‐Liss, Inc.