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Characterization of the chitin biosynthesis process as a compensatory mechanism in the fks1 mutant of Saccharomyces cerevisiae

✍ Scribed by L.J Garcı́a-Rodriguez; J.A Trilla; C Castro; M.H Valdivieso; A Durán; C Roncero

Book ID
117102111
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
331 KB
Volume
478
Category
Article
ISSN
0014-5793

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The mutant tmpl--10ts which confers thermosensitive auxotrophy for thymidylate is employed for the selection of 5'-dTMP uptaking mutants. At the nonpermissive temperature yeast cells phenotypically wild type for thymidylate uptake can grow for only 3 to 4 generations in the presence of 10(-2) M 5'-d