Characterization of the chemotactic and mitogenic response of SMCs to PDGF-BB and FGF-2 in fibrin hydrogels

✍ Scribed by Areck A. Ucuzian; Luke P. Brewster; Andrea T. East; Yongang Pang; Andrew A. Gassman; Howard P. Greisler

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 623 KB
• Volume: 9999A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.32786

✦ Synopsis

Abstract

The delivery of growth factors to cellularize biocompatible scaffolds like fibrin is a commonly used strategy in tissue engineering. We characterized smooth muscle cells (SMC) proliferation and chemotaxis in response to PDGF‐BB and FGF‐2, alone and in combination, in 2D culture and in 3D fibrin hydrogels. While both growth factors induced an equipotent mitogenic response in 2D culture, only FGF‐2 was significantly mitogenic for SMCs in 3D culture. Only PDGF‐BB was significantly chemotactic in a modified Boyden chamber assay. In a 3D assay of matrix invasion, both growth factors induced an invasive response into the fibrin hydrogel in both proliferating and nonproliferating, mitomycin C (MMC) treated cells. The invasive response was less attenuated by the inhibition of proliferation in PDGF‐BB stimulated cells compared with FGF‐2 stimulated cells. We conclude that SMCs cultured in fibrin hydrogels have a more robust chemotactic response to PDGF‐BB compared with FGF‐2, and that the response to FGF‐2 is more dependent on cell proliferation. Delivery of both growth factors together potentiates the chemotactic, but not mitogenic response to either growth factor alone. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010