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Characterization of The Active Site of Schwanniomyces Occidentalis Glucoamylase by In Vitro Mutagenesis

✍ Scribed by Birgit Hülseweh; Ulrike M. Dahlems; Jürgen Dohmen; Alexander W. M. Strasser; Cornelis P. Hollenberg


Book ID
115132355
Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
619 KB
Volume
244
Category
Article
ISSN
1432-1327

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To better understand the molecular basis of glucomylase selectivity, lowenergy conformers of glucosyl disaccharides obtained from relaxed-residue conformational mapping were flexibly docked into the glucoamylase active site using AutoDock 2.2. This procedure ensures that significant conformational s