A method was devised for assaying protein kinases that phosphorylate either Kemptide, such as cAMP-dependent protein kinase, or a glycogen synthase peptide, which is an excellent substrate for protein kinase C. Upon sequential processing of reaction mixtures through tandem columns of cation and anio
Characterization of Synthetic Peptide Substrates for p34cdc2 Protein Kinase
β Scribed by Daniel R. Marshak; Mark T. Vandenberg; Young Seuk Bae; Ii Je Yu
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 888 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0730-2312
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β¦ Synopsis
Synthetic peptide substrates for the cell division cycle regulated protein kinase, ~34'~'', have been developed and characterized. These peptides are based on the sequences of two known substrates of the enzyme, Simian Virus 40 Large T antigen and the human cellular recessive oncogene product, p53.
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