Characterization of spontaneous cell death in monolayer cultures of primary hepatocytes
โ Scribed by Mathieu Vinken; Elke Decrock; Tatyana Doktorova; Eva Ramboer; Elke De Vuyst; Tamara Vanhaecke; Luc Leybaert; Vera Rogiers
- Publisher
- Springer-Verlag
- Year
- 2011
- Tongue
- English
- Weight
- 404 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0340-5761
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## Abstract Mitochondrial respiration is inhibited in cells exposed to hypoxia, and the oxidation of NADH to NAD^+^ is blocked. As a result, oxidation reactions requiring NAD^+^ are blocked, disrupting cellular metabolism. We studied the influence of methylene blue, which oxidizes NADH, on hypoxic