Characterization of Protein Impurities and Site-Specific Modifications Using Peptide Mapping with Liquid Chromatography and Data Independent Acquisition Mass Spectrometry

Rapid characterization of covalent modif

Rapid characterization of covalent modifications to rat brain mitochondrial proteins after ex vivo exposure to 4-hydroxy-2-nonenal by liquid chromatography–tandem mass spectrometry using data-dependent and neutral loss-driven MS3 acquisition

✍ Stanley M. Stevens Jr; Navin Rauniyar; Laszlo Prokai 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 267 KB

## Abstract The modification of mitochondrial proteins enriched from rat forebrain by the major lipid peroxidation product 4‐hydroxy‐2‐nonenal (HNE) was investigated using high performance liquid chromatography (HPLC) and tandem mass spectrometry. Subcellular fractionation in conjunction with a ‘sh