Characterization of prostaglandin F2α receptor of mouse 3T3 fibroblasts and its functional expression in Xenopus laevis oocytes

✍ Scribed by Akihide Nakao; Tsuyoshi Watanabe; Shigeo Taniguchi; Motonao Nakamura; Zen-Ichiro Honda; Takao Shimizu; Kiyoshi Kurokawa

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 824 KB
• Volume: 155
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041550206

✦ Synopsis

Prostaglandin (PG) F , , increased [3H]thymidine incorporation into quiescent NIH 3T3 cells, stimulated phosphoinositide breakdown, and raised intracellular Ca2+ concentration ([Ca2*],) in a dose-dependent manner with ED,, values of 2.0 X l o p 8 M, 4.6 X M, and 7.5 X l o p 8 M, respectively. The increase in [3H]thymidine incorporation with PGF,, was additive with that seen with epidermal growth factor (EGF) or insulin. The peak 1Ca2'], increase with PGF, , was still obvious in the absence of extracellular Ca2+ and was insensitive to islet activating protein (IAP) pretreatment. Membranes prepared from NIH 3T3 cells exhibited a specific binding for PGF,,, which was sensitive to GTP,S but not sensitive to IAP pretreatment. Xenopus laevis oocytes injected with NIH 3T3 cell mRNA between 185 and 28s rRNA fractionated by sucrose gradient, expressed a PGF,,-specific CIcurrent when examined by voltage clamp. This CI-current was also insensitive to IAP pretreatment and not affected by extracellular Ca'+ concentration ([Ca2'],).

These results indicate 1 ) that the NIH 3T3 cells expressed a specific PGF, ,