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Characterization of plasmid burden and copy number in Saccharomyces cerevisiae for optimization of metabolic engineering applications

✍ Scribed by Karim, Ashty S.; Curran, Kathleen A.; Alper, Hal S.


Book ID
118739620
Publisher
John Wiley and Sons
Year
2013
Tongue
English
Weight
365 KB
Volume
13
Category
Article
ISSN
1567-1356

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πŸ“œ SIMILAR VOLUMES


Effect of plasmid size and medium on gro
✍ S. J. Coppella; P. Dhurjati πŸ“‚ Article πŸ“… 1989 πŸ› Springer 🌐 English βš– 599 KB

Saccharomyces cerevisiae cultures were selected to determine the effects of plasmid size on host growth kinetics and plasmid copy number. A complete synthetic medium was verified and the effects of yeast nitrogen base without amino acids medium and leucine selection were established for the strain.

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Autoselection systems allow the selection of a genetically engineered population independently of the growth medium composition. The structure of a Saccharomyces cerevisiae population transformed with an autoselection plasmid, in which a carbon-source-dependent modulation of the plasmid copy number