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Characterization of PicoGreen Reagent and Development of a Fluorescence-Based Solution Assay for Double-Stranded DNA Quantitation

✍ Scribed by Victoria L. Singer; Laurie J. Jones; Stephen T. Yue; Richard P. Haugland

Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
237 KB
Volume
249
Category
Article
ISSN
0003-2697

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Various assays are available for quantification of DNA in solution, but none has been described that is both sensitive and specific for double-stranded (ds) DNA and features practical properties such as low dye and equipment costs, speed, and highly parallel microplate formats. Here we show that qua