Characterization of phenolic glucosides from soybean root nodules by ion-exchange high performance liquid chromatography, ultraviolet spectroscopy and electrospray mass spectrometry
✍ Scribed by Jose F. Moran; Robert V. Klucas; Renée J. Grayer; Joaquin Abian; Jeffrey B. Harborne; Manuel Becana
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 117 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0958-0344
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✦ Synopsis
Ion-exchange high performance liquid chromatography (HPLC) with an Aminex column and photodiodearray detection was used to purify, from soybean root nodules, highly polar phenolic acid conjugates that are recalcitrant to isolation by conventional techniques based on reversed-phase chemistry. HPLC, acid and enzymatic hydrolysis, ultraviolet spectroscopy, high resolution fast atom bombardment mass spectrometry (MS) and single and tandem electrospray MS were used to identify the 4-O-b-glucosides of phydroxybenzoic, protocatechuic and vanillic acids (which are reported in legumes for the first time) and the 5-O-b-glucoside of gentisic acid. The four phenolic acids, as well as gallic acid and its methyl ester, were also found in soybean nodules.