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Characterization of peroxidases in luminol chemiluminescence coupled with copper-catalysed oxidation of cysteamine

✍ Scribed by Kamidate, Tamio ;Katayama, Atsushi ;Ichihashi, Hiroyuki ;Watanabe, Hiroto

Publisher: John Wiley and Sons
Year: 1994
Weight: 626 KB
Volume: 9
Category: Article
ISSN: 0884-3996
DOI: 10.1002/bio.1170090406

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✦ Synopsis

Hydrogen peroxide formed during the course of the copper(l1)-catalysed oxidation o f cysteamine with oxygen was continuously determined by a peroxidase (POD)-catalysed luminol chemiluminescence (CL) method. Horseradish peroxidase (HRP), lactoperoxidase (LPO) and Arthromyces ramosus peroxidase (ARP) were used as a CL catalyst. The respective PODs gave specific CL intensity-time profiles. HRP caused a CL delay, and ARP gave a time-response curve which followed the production rate of H202. LPO gave only a weak CL flash which decayed promptly. These differences o f CL response curves could be explained in terms o f the different reactivities of PODs for superoxide anion and the different formation rate o f luminol radicals in the peroxidation of luminol catalysed by POD.

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