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Characterization of one- and two-photon excitation fluorescence resonance energy transfer microscopy

✍ Scribed by Masilamani Elangovan; Horst Wallrabe; Ye Chen; Richard N. Day; Margarida Barroso; Ammasi Periasamy

Book ID
117702193
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
912 KB
Volume
29
Category
Article
ISSN
1046-2023

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## Abstract Two‐photon excitation fluorescence resonance energy transfer (2P‐FRET) imaging microscopy can provide details of specific protein molecule interactions inside living cells. Fluorophore molecules used for 2P‐FRET imaging have characteristic absorption and emission spectra that introduce