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Characterization of nitrogen-fixing (C2h2-reducing) Bacillus species from Egyptian soils

โœ Scribed by Dr. A. M. Abdel Wahab


Book ID
102909373
Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
534 KB
Volume
20
Category
Article
ISSN
0233-111X

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โœฆ Synopsis


The characteristics of ten N,(C,H,)-fixing Bacillus strains, isolated from Egyptian soils. were investigated. The isolates were not completely identical to any of the described species in the 8th Edition of BERGEY'S Manual. However, two strains were closely related to B. cereus, five strains were tentatively characterized as B. lichenqormis and three strains with swollen sporangia and thick-walled endospores shared their main characteristics with B. circulans.

A comparison between these bacilli and other known nitrogen-fixing Bacillus species revealed major differences in culture characteristics and C,H,-reducing activities. This suggests that the Bacillus isolates described here are new nitrogen-fixing bacteria.


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Twenty out of 400 isolates of bacteria mainly from salt marshes and saline soils of Egypt were successfully grown on mannitol N-free medium. The nitrogen-fixing activity was then demonstrated for the twenty isolates in modified STANIER'S medium using the acetylene reduction assay. All of them posses