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Characterization of neural cell adhesion molecules (NCAM) expressed by ewing and neuroblastoma cell lines

โœ Scribed by Marc Lipinski; Marie-Rose Hirsch; Hermine Deagostini-Bazin; Osamu Yamada; Thomas Tursz; Christo Goridis

Publisher
John Wiley and Sons
Year
1987
Tongue
French
Weight
948 KB
Volume
40
Category
Article
ISSN
0020-7136

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โœฆ Synopsis

Marie-Rose HIRSCH', Hermine DEAGOSTINI-BAZIN', Osamu YAMADA' , Thomas TURSZ' and The status of the neural cell adhesion molecule N C A M gene which is mapped to human chromosome llq23-24 has been investigated in Ewing-tumor-derived cell lines which present the t(l I ;22)(q23-24;q12) translocation characteristic of this malignancy. N o rearrangement was detected when 2 different non-overlapping probes to mouse N C A M were used. The expression of the N C A M gene was analysed at both the protein and messenger levels in material extracted from Ewing cell lines, human neuroblastoma cell line and fetal mouse brain. Immune blot and immunoprecipitation studies showed that the neuroblastoma cell line contained more NCAM material than the Ewing lines. In neuroblastoma but not in Ewing, the N C A M material had the electrophoretic characteristics of molecules with long polysialic acid chains. After treatment with endosialidase, the diffusely migrating neuroblastoma material was resolved into 3 discrete bands of 120, I40 and 180 kDa. In Ewing extract, high-molecular-weight N C A M species were also detected with a 3-band pattern more reminiscent of mature brain. Endoglycosidase F treatment of Ewing N C A M indicated that all 3 species were largely Nglycosylated. Northern blot analysis confirmed that NCAM was expressed more abundantly in neuroblastoma than in Ewing cell lines. Among the 4 N C A M messengers (7.0.6.5, 4.3 and 4. I kb) detected in the neuroblastoma, the 6.5 kb species was largely predominant. The Ewing messenger RNA pattern was clearly different :as the largest 7.0-kb species was virtually absent and the other bands were of similar intensities.

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