Characterization of mutant Ha-ras gene expression in transformed murine keratinocyte lines grown under in vitro and in vivo conditions
✍ Scribed by John P.G. Volpe; Claudio J. Conti; Thomas J. Siaga
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 484 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0899-1987
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✦ Synopsis
We investigated the Ha-ras and Ki-ras gene status, tumorigenicity, pathology, line derivation, and intercellular communication of 7,12-dimethylbenz[a]anthracene-initiated papilloma-, carcinoma-, and hyperplastic skinproducing cell lines to further characterize them. Six of nine tumor cell lines grown in vitro expressed both mutant and normal Ha-ras proteins, and three lines expressed only normal Ha-ras. However, when grown subcutaneously in nude mice, seven of the nine lines expressed both mutant and normal Ha-ras, one line expressed normal Ha-ras, and one line did not grow subcutaneously. One papilloma line, PU15, appeared to have an inducible mutant Ha-ras gene, as it was expressed only in vivo. These findings suggest that mutant Haras genes may be lost in only a minor population of tumor lines during growth in culture. Finally, we found that mutant Ha-ras gene expression was strongly correlated with tumorigenicity in nude mice and that intercellular communication was strongly correlated with the derivation of the lines.