𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Characterization of monoclonal antibodies directed against distinct conserved epitopes of human immunodeficiency virus type 1 core proteins

✍ Scribed by Masashi Tatsumi; Christian Devaux; François Kourilsky; Jean-Claude Chermann

Publisher
Springer
Year
1990
Tongue
English
Weight
688 KB
Volume
96
Category
Article
ISSN
0300-8177

No coin nor oath required. For personal study only.

✦ Synopsis

Two mouse hybridoma cell lines secreting antibodies to the Human Immunodeficiency Virus (HIV) p25 major core protein and its precursors p55 and p41, were developed after immunization with the highly cytopathic Zaïrian HIV-1 isolate, NDK. These monoclonal antibodies also react with the gag gene products from HIV-1-BRU prototype and present cross reaction with HIV-2-ROD, and SIV-AGM. They map into topographically distinct areas of p25 and define epitopic regions topographically separated from those recognized by four other anti-p25 mAb suggesting the existence of at least 6 spatially distinct epitopic regions on HIV-1-p25 core protein.

📜 SIMILAR VOLUMES

Characterization of monoclonal antibodie
Characterization of monoclonal antibodies identifying type and strain-specific epitopes of human immunodeficiency virus type 1
✍ Véronique Robert; Mariana Resnicoff; Jean-Claude Chermann; Christian Devaux 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 669 KB

Several hybridoma cell lines were raised against the highly cytopathic Zaïrian isolate of Human Immunodeficiency Virus (HIV), HIV1-NDK. The specificity of the secreted monoclonal antibodies (mAb) was demonstrated by immunoblotting, radioimmunoprecipitation and immunofluorescence. Two hybridoma cell

Generation and characterization of monoc
Generation and characterization of monoclonal antibodies against multiple epitopes on the C-terminal half of envelope gp46 of human T-cell leukemia virus type-I (HTLV-I)
✍ Yuetsu Tanaka; Masazumi Yasumoto; Hiroshi Nyunoya; Tsutomu Ogura; Masayoshi Kiku 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 French ⚖ 1000 KB

In order to study the antigenicity of envelope 46 kDa glycoprotein (gp46) of human T-cell leukemia virus type-I (HTLV-I), we have generated monoclonal anti-gp46 antibodies (MAbs), REY-7, REY-I I, REY-16, REY-30, MET4 and MET-3 from rats and mice. lmmunoblot and immunofluorexence assays showed that t

Identification of the core residues of t
Identification of the core residues of the epitope of a monoclonal antibody raised against glycoprotein D of herpes simplex virus type 1 by screening of a random peptide library
✍ Gerard A. Schellekens; Edwin Lasonder; Matty Feijlbrief; Danny G. A. M. Koedijk; 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 686 KB

Identification of the core residues of the epitope of a monoclonal antibody raised against glycoprotein D of herpes simplex virus type 1 by screening of a random peptide library\* Random peptide libraries (RPL) displayed on the surface of a filamentous bacteriophage can be used to identify peptide l

Production of a recombinant human T-cell
Production of a recombinant human T-cell leukemia virus type-I trans-activator (tax1) antigen and its utilization for generation of monoclonal antibodies against various epitopes on the tax1 antigen
✍ Yuetsu Tanaka; Atsushi Yoshida; Hideki Tozawa; Hisatoshi Shida; Hiroshi Nyunoya; 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 French ⚖ 967 KB

A 42-kDa recombinant protein, PX141, consisting of the trans-activator protein encoded by human T-cell leukemia virus (HTLV-I) (tax, antigen) and the amino-terminal fusion peptide of I 2 amino acid residues of the a-peptide encoded by the plasmid pUC I 9 was produced. In order to investigate the imm