Characterization of modification sites during peptide synthesis using liquid secondary ion/collision-induced dissociation mass spectrometry and a computer program
✍ Scribed by Jef Rozenski; Isabelle Samson; Gerard Janssen; Roger Busson; Arthur Van Aerschot; Piet Herdewijn
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 446 KB
- Volume
- 29
- Category
- Article
- ISSN
- 1076-5174
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✦ Synopsis
Side-reactions often occur during peptide synthesis resulting in modified amino acid moieties. To identify these residues, liquid secondary ion/collision-induced dissociation mass spectra were recorded. The main fragments are generated by cleavage of the peptide bond. To facilitate interpretation of the spectra and assignment of the structure, a simple, but flexible and efficient, computer program is presented. The program allows the verification of the correct structure of the synthesized peptides and the deduction of the type of side-products formed, such as alkylation of tryptophan residues.