Characterization of metastable intermediates of enzymatic peroxidation of NAD+ by on-line capillary electrophoresis/electrospray ionization mass spectrometry

✍ Scribed by Zhao, Zhongxi (Zack); Udseth, Harold R.; Smith, Richard D.

• Book ID: 102646208
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 517 KB
• Volume: 31
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/(sici)1096-9888(199602)31:2<193::aid-jms282>3.0.co;2-7

✦ Synopsis

The unstable intermediate products formed from the degradation of nicotinamide adenine dinucleotide (NAD') catalyzed by the reaction of equine liver alcohol dehydrogenase with hydrogen peroxide were studied. Reversed anionic capillary electrophoresis (CE)/electrospray ionization mass spectrometry (ESI-MS) was used to separate the reaction mixture and to monitor the reaction process, and to provide both molecular weight and structural information for the interaction intermediates. The mass spectra of the reartion intermediates provide support for the reaction mechanism previously proposed by Favilla et al. The present results also indicate that a different reaction intermediate may also be involved in the interaction, and contributes to the reaction. The results illustrate the utility of combined C E and ESI-MS for the on-line study of biochemical processes.