Characterization of meiosis-deficient mutants by electron microscopy and mapping of four essential genes in the fission yeast Schizosaccharomyces pombe
✍ Scribed by Shimoda, Chikashi ;Hirata, Aiko ;Kishida, Masao ;Hashida, Takashi ;Tanaka, Kenji
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 697 KB
- Volume
- 200
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
Meiosis-deficient mutants of the fission yeast
Schizosaccharomyces pombe carrying meil, mei2, mei3, mei4 and mesl mutant alleles were characterized by electron microscopy and staining of the nucleus with 4', 6-diamidino-2phenylindole. Zygotes of either meil, mei2 or mei3 mutants contained one round nucleus with a single spindle pole body (SPB). These mutants were arrested before premeiotic DNA synthesis. Zygotes of mei4 mutants had one elongated nucleus containing thick electron-dense filaments (linear elements). In the mesl mutant, the first meiotic division was completed but the SBPs did not duplicate. Modification of the SPB (outer plaque formation) was also blocked and the forespore membrane was not assembled.
By haploidization, random spore and tetrad analyses, four essential genes for meiosis (mei2, mei3, mei4 and mesl) were mapped. Gene mei2 was located on chromosome I 14.2 cM distant from ura2. Gene mei3 was linked to ade7 (45.4 cM) on chromosome II. Gene mei4 was linked to cde2 (0.6 cM) on chromosome II. Gene mesl was linked to ura3 (25.3 cM) on chromosome I.