Characterization of lymphoid cells in the blood of healthy adults: Sequential immunological, cytochemical and cytokinetic studies

✍ Scribed by A. Hirt; H. P. Wagner

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: English
• Weight: 594 KB
• Volume: 9
• Category: Article
• ISSN: 0361-8609
• DOI: 10.1002/ajh.2830090309

✦ Synopsis

Abstract

With a new method, sequential immunological, cytochemical and cytokinetic studies were done on lymphoid cells in the peripheral blood of 12 healthy adults. Every single lymphoid cell could therefore be characterized by the following markers: surface immunoglobulins (sIg); rosetting with sheep red blood cells (E); unspecific acid alpha‐naphthyl acetate esterase (ANAE); and ^3^HdT incorporation. Significantly more E^+^sIg^−^ cells were ANAE^+^ than E^−^sIg^+^ cells (70% vs. 11%). About half of the E^+^sIg^−^ cells were ANAE^+^. By combining esterase and surface marker analyses eight subpopulations of lymphoid cells could be distinguished. The most common subpopulations were E^+^sIg^−^ ANAE^+^ and E^+^sIg^−^ANAE^−^ cells (51% and 22% of all lymphoid cells, respectively). Of all ANAE^+^ cells 90% were E^+^, but 64% of all ANAE^−^ cells were also E^+^. In all individuals a subpopulation of E^+^sIg^+^ cells was found. The esterase pattern of these cells was similar to that of E^−^sIg^+^ cells. The overall labeling index of the lymphoid cells examined was ≦ 0.2%.