Characterization of intracellular calcium mobilization by bombesin-related neuropeptides in PC-3 human prostate cancer cells

✍ Scribed by Han, Kehan; Viallet, Jean; Chevalier, Simone; Zheng, Wei; Bazinet, Michel; Aprikian, Armen G.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 302 KB
• Volume: 31
• Category: Article
• ISSN: 0270-4137
• DOI: 10.1002/(sici)1097-0045(19970401)31:1<53::aid-pros9>3.0.co;2-j

✦ Synopsis

Background:

The objective of this study was to characterize the calcium mobilizing activity of bombesin-related neuropeptides in pc-3 cells.

Methods:

Intracellular free ca2+ concentration ([ca2+]i) was measured following the administration of bombesin-related neuropeptides using spectrofluorometric monitoring of pc-3 cells loaded with fura2/am.

Results:

Individual neuropeptides induced a rapid rise in [ca2+]i immediately after stimulation in similar fashion. when two different peptides were administered simultaneously, the [ca2+]i changes were similar to that produced by each peptide alone. however, simultaneous addition of tyr4-bombesin 100 nm and 2% serum resulted in [ca2+]i increase greater than either alone. sequential stimulation by same or different peptides, was not able to induce another response. however, 6 hrs after the first stimulus, a second stimulus was able to induce a similar response.

Conclusions:

The data indicate that all members of the bombesin-related family of neuropeptides are capable of triggering calcium mobilization in pc-3 cells via a similar signalling pathway. pc-3 cells become acutely desensitized to additional stimulation of neuropeptide. this desensitization is selective and reversible, requiring approximately 6 hrs for recovery.