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Characterization of intact microorganisms by MALDI mass spectrometry

✍ Scribed by Catherine Fenselau; Plamen A. Demirev

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
210 KB
Volume
20
Category
Article
ISSN
0277-7037

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

| I. | Introduction | 157 |
| II. | MALDI‐TOF Spectra of Biomarkers | 158 |
| III. | Instrumentation | 158 |
| IV. | Sample Introduction | 160 |
| V. | Preparation of Microorganism Samples for MALDI Analysis | 161 |
| VI. | Characterization of MALDI Biomarkers | 162 |
| VII. | Data Analysis: Fingerprint Libraries, Statistical Methods, Bioinformatics | 163 |
| VIII. | Applications | 165 |
| IX. | Summary and Envoi | 166 |
| Acknowledgments | 166 |
| References | 166 |

The application of MALDI mass spectrometry to desorb protein biomarkers from intact viruses, bacteria, fungus, and spores is the focus of this review. Instrumentation, sample collection, sample preparation, and algorithms for data analysis are summarized. Optimally these analyses should be carried out in less than five minutes. Successful applications are discussed from biotechnology, cell biology, and the pharmaceutical industry. Β© 2002 John Wiley & Sons, Inc., Mass Spec Rev 20:157–171, 2001

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