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Characterization of immunoglobulin mrna expression in burkitt lymphoma cell lines

✍ Scribed by Roberto Anker; James Caldwell; Jane Brokaw; Brian A. Pollok

Publisher
John Wiley and Sons
Year
1989
Tongue
French
Weight
912 KB
Volume
43
Category
Article
ISSN
0020-7136

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✦ Synopsis

Immunoglobulin heavy-and light-chain mRNA of I I Burkitt lymphoma (BL) cell lines (9 African and 2 American) were analyzed for various structural characteristics. In agreement with previous results at the protein level, all the BIL cell lines express heavy-chain mRNA transcripts of the p class. Surprisingly, a high p&., mRNA ratio was found in 2 IgM-producing BL cell lines (Raji and CCL85). that do not secrete immunoglobulin. Variable region gene use was also assessed in the cell lines: while 4 out of 7 endemic BL cell lines use V, genes that belong to the V,3 gene family, no clear bias in the expression of particular V, or V, gene families among this sampling of BL lines was found. Northern blot analysis of immurioglobulin transcripts in endemic BL cell lines did show that 2 such lines (AG876 and HT862) expressed truncated heavy-chain transcripts; RNA sequence analysis of the V, region demonstrated different abnormal 5'-localized RNA spliciing events for the 2 shortened p transcripts. The light-chain mRNA in these 2 cell lines also showed structural abnormalities and, in the case of HTB62, 3 different K light-chain transcripts are produced (of elongated, native and truncated sizes:). In vitro translation of mRNA from HTB62 showed p and K chain proteins corresponding with the relative size for each message.

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