Characterization of hydroxyaromatic compounds in vegetable oils by capillary electrophoresis with direct injection in an oil-miscible KOH/propanol/methanol medium
✍ Scribed by Carla R. B. Mendonça; Clara I. D. Bica; Clarisse M. S. Piatnicki; Ernesto F. Simó-Alfonso; Guillermo Ramis-Ramos
- Book ID
- 102184514
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 167 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
Abstract
The separation of hydroxyaromatic compounds in vegetable oils, including synthetic antioxidants (3‐tert‐butyl‐4‐hydroxyanisol and 2,6‐di‐tert‐butyl‐4‐hydroxytoluene), E‐vitamers and other natural oil components, by nonaqueous capillary electrophoresis in an oil‐miscible background electrolyte (BGE) was investigated. The BGE contained 40 mM KOH in a methanol/1‐propanol (PrOH) mixture (15:85 v/v). The oil samples were 1:1 diluted with PrOH and directly injected in the capillary. Under negative polarity (cathode at the injection end), the anionic solutes moved faster than the electroosmotic flow, being well‐resolved among them and from the triacylglycerols. Using virgin palm, extra virgin olive, wheat germ, virgin soybean and other oils, the capability of the procedure to quickly yield a characteristic profile of the biophenols present in the sample was demonstrated. The α‐, (β+ γ)‐ (as unresolved pair) and δ‐tocopherols of a soybean oil sample were quantified.