✍ Scribed by Tibor Bakæcs; Peter Gergely; Santoso Cornain; Eva Klein
No coin nor oath required. For personal study only.
Lymphocyte fractions of three healthy donors were tested for cytotoxicity in a 48‐h assay on several tumor‐derived lines. Analysis of surface markers indicated that a non‐T non‐B fraction comprising about 50% Fc‐receptor‐positive cells was most potent. The cell yield in this fraction was on the average 6.5% of the non‐fractionated population. Elimination of SIg positive cells did not influence the cytotoxic potential. Pure T cells isolated with E‐rosetting subsequent to passage on nylon wool column had no or very low cytotoxicity. We had indications that cells without conventional markers —“null” cells—were also cytotoxic.
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## Abstract The effect of serum on the K562 assay for spontaneous human lymphocyte‐mediated cytotoxicity (SLMC) was examined systematically at the level of the assay system and with respect to the serum used for the long‐term maintenance of the K562 target cells. It was found that the assay did not