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Characterization of HSCD5, a novel human stearoyl-CoA desaturase unique to primates

✍ Scribed by Jian Wang; Lan Yu; Robert E. Schmidt; Chen Su; Xiaodi Huang; Kenneth Gould; Guoqing Cao


Book ID
116290612
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
822 KB
Volume
332
Category
Article
ISSN
0006-291X

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A segment of 712 bases coding for part of the human stearoyl-CoA desaturase gene was made by polymerase chain reaction (PCR) using primers based on published rat cDNA sequences. The human PCR product was confirmed by DNA sequencing. It was next cloned into a vector from which anti-sense, highly radi